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KMID : 0811720160200020169
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Korean Journal of Physiology & Pharmacology
2016 Volume.20 No. 2 p.169 ~ p.175
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High glucose and palmitate increases bone morphogenic protein 4 expression in human endothelial cells
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Hong Oak-Kee
Yoo Soon-Jib
Son Jang-Won
Kim Mee-Kyoung
Baek Ki-Hyun
Song Ki-Ho
Cha Bong-Yun
Jo Han-Joong
Kwon Hyuk-Sang
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Abstract
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Here, we investigated whether hyperglycemia and/or free fatty acids (palmitate, PAL) aff ect the expression level of bone morphogenic protein 4 (BMP4), a proatherogenic marker, in endothelial cells and the potential role of BMP4 in diabetic vascular complications. To measure BMP4 expression, human umbilical vein endothelial cells (HUVECs) were exposed to high glucose concentrations and/ or PAL for 24 or 72 h, and the effects of these treatments on the expression levels of adhesion molecules and reactive oxygen species (ROS) were examined. BMP4 loss-of-function status was achieved via transfection of a BMP4-specific siRNA. High glucose levels increased BMP4 expression in HUVECs in a dose-dependent manner. PAL potentiated such expression. The levels of adhesion molecules and ROS production increased upon treatment with high glucose and/or PAL, but this effect was negated when BMP4 was knocked down via siRNA. Signaling of BMP4, a proinfl ammatory and pro-atherogenic cytokine marker, was increased by hyperglycemia and PAL. BMP4 induced the expression of infl ammatory adhesion molecules and ROS production. Our work suggests that BMP4 plays a role in atherogenesis induced by high glucose levels and/or PAL.
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KEYWORD
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Bone morphogenic protein 4, Diabetes mellitus, Free fatty acid, High glucose
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